alpha-Latrotoxin Protein

Catalog No. ABIN7582344

This Native alpha-Latrotoxin protein is expressed in Spider (Araneae).

Quick Overview for alpha-Latrotoxin Protein (ABIN7582344)

Target: alpha-Latrotoxin

Protein Type: Native

Origin: Latrodectus tredecimguttatus

Source: Spider (Araneae)

Application: Blocking Peptide (BP)

Purity: ≥98 % (HPLC)

Product Details

Purpose: An Inducer of Neurotransmitter Release

Specificity: Toxin target: Neurexin-1, ADGRL1 (latrophilin-1/CIRL), and tyrosine-protein phosphatase S (PTPRS) receptors, Highly disulfide bridged protein Glycosylated

Characteristics: An Inducer of Neurotransmitter Release

Application Details

Application Notes: Antigen Preadsorption Control: 150 pM - 1 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. Soluble in water. Once dissolved in water, add an equal volume of glycerol. Do not shake or vortex. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyopholized in 20 mM KPO4 pH 6.8.

Storage: 4 °C,-20 °C

Storage Comment: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

Target Details

Target: alpha-Latrotoxin

Background: α-Latrotoxin-Lt1a, α-LTX-Lt1a, α-LTX,α-Latrotoxin is a 130 kDa protein toxin from the black widow spider venom and is the only protein in the venom that affects mammals.1,2 Application of the toxin to presynaptic preparations induces, after a delay, a huge increase in spontaneous neurotransmitter release, which can be evaluated by measuring the post synaptic response in the form of miniature end plate potentials. This toxin is widely used to induce and study neurotransmitter release, but the molecular mechanism of its action is not fully determined.α-Latrotoxin is isolated according to a modified protocol as described by Frontali3 and Grasso4.

Molecular Weight: 130 kDa

UniProt: P23631