Muscarinic Toxin 3 Protein

Catalog No. ABIN7822037

This Native Muscarinic Toxin 3 protein is produced in Snake (Serpentes).

Quick Overview for Muscarinic Toxin 3 Protein (ABIN7822037)

Target: Muscarinic Toxin 3

Protein Type: Native

Origin: Dendroaspis angusticeps

Source: Snake (Serpentes)

Application: Blocking Peptide (BP)

Purity: >99 % (HPLC)

Product Details

Purpose: An Antagonist of M1 and M4 Muscarinic Receptors and Adrenoceptors

Sequence: LTCVTKNTIF GITTENCPAG QNLCFKRWHY VIPRYTEITR GCAATCPIPE NYDSIHCCKT DKCNE-OH

Specificity: Toxin target: M1, M4 muscarinic receptors, adrenoceptors,
Disulfide bonds between: Cys3-Cys24, Cys17-Cys42, Cys46-Cys57and Cys58-Cys63

Characteristics: An Antagonist of M1 and M4 Muscarinic Receptors and Adrenoceptors

Application Details

Application Notes: Antigen Preadsorption Control: 100 nM - 1 μM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage: 4 °C,-20 °C

Storage Comment: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Target Details

Target: Muscarinic Toxin 3

Background: MT3, MT-3,Muscarinic toxins are isolated from the venom of the mamba species and are composed of 65 to 66 amino acid residues with four intramolecular disulfide bridges. One of these is muscarinic toxin 3 (MT-3) which was isolated from the green mamba (Dendroaspis augusticeps) and is composed of 65 amino acid residues1. A recent study investigated the interaction of MT3 with cloned receptors (mAChRs and adrenoceptors) expressed in insect cells by radioligand binding. MT3 appears to have a broad spectrum of targets showing high-affinity binding (IC50 = 1-10 nM) to M4 mAChR, α1A-, α1D- and α2A-adrenoceptors and lower affinity binding (IC50 > 25 nM) to α1B- and α2C-adrenoceptors and M1 mAChR2.

Molecular Weight: 7379 Da

UniProt: P81031