PLTX-II Protein

Catalog No. ABIN7582350

This Native PLTX-II protein is expressed in Spider (Araneae).

Quick Overview for PLTX-II Protein (ABIN7582350)

Target: PLTX-II

Protein Type: Native

Origin: Plectreurys tristis

Source: Spider (Araneae)

Application: Blocking Peptide (BP)

Purity: ≥99 % (HPLC)

Product Details

Purpose: An Inhibitor of CaV Channels

Sequence: ADCSATGDTC DHTKKCCDDC YTCRCGTPWG ANCRCDYYKA RCDT-NH2

Specificity: Toxin target: CaV Ca2+ channels,
Disulfide bonds between: Cys3-Cys17, Cys10-Cys23, Cys16-Cys35, Cys20-Cys42 and Cys25-Cys33 Thr44 - C-terminal amidation and Opalmitoylation

Characteristics: An Inhibitor of CaV Channels

Application Details

Application Notes: Antigen Preadsorption Control: 10 - 50 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized Powder.

Storage: 4 °C,-20 °C

Storage Comment: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Target Details

Target: PLTX-II

Background: ω-Plectoxin-Pt1a, ω-PLTX-Pt1a, PLTX-2,Plectreurys toxin (PLTX-II) is a 44 amino acid peptidyl toxin produced by Plectreurys tristis spider1,2. PLTXII was found as a potent blocker of the CaV2 channel (CAC) that inactivates Ca2+ currents in pupal neurons3 and in antennal lobe PNs of adult Drosophila brain. PLTX-II regulates the miniature excitatory postsynaptic current frequency at excitatory cholinergic synapses of these neurons in vitro and in situ and acts as a presynaptic Ca2+-channel blocker3,4.

Molecular Weight: 5108.7 Da

UniProt: P34079