Tetanus Toxin Protein (TetX)

Catalog No. ABIN7582361

This Native Tetanus Toxin protein is produced in Clostridium tetani.

Quick Overview for Tetanus Toxin Protein (TetX) (ABIN7582361)

Target: Tetanus Toxin (TetX)

Protein Type: Native

Origin: Clostridium tetani

Source: Clostridium tetani

Application: Blocking Peptide (BP)

Purity: ≥97 % (HPLC)

Product Details

Purpose: An Inhibitor of Neurotransmitter Release

Specificity:
Disulfide bonds between Cys439-Cys467 and Cys1077-Cys1093

Characteristics: An Inhibitor of Neurotransmitter Release

Application Details

Application Notes: Antigen Preadsorption Control: 1 - 100 nM

Comment:

Electrophysiology

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. Soluble in any conventional buffer containing 0.01 KH2PO4, 0.1 % gelatin and/or 0.1 % bovine serum albumin (BSA). The use of gelatin or 0.01 % BSA coated/treated glassware is recommended. Centrifuge all product preparations before use (10000 x g 5 min).

Buffer: Lyophilized Powder.

Storage: 4 °C,-20 °C

Storage Comment: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Target Details

Target: Tetanus Toxin (TetX)

Alternative Name: Tetanus Toxin

Target Type: Bacteria

Background: Tentoxylysin,Tetanus toxin is a 150 kD protein produced by the gram positive bacteria Clostridium tetani under anaerobic condition1. Tetanus toxin is a hetrodimer of A-chain (50 kD) and B-chain (100 kD) linked by one disulfide bond2,3. The A-chain has a zinc endopeptidase activity while the B-chain has a role in toxin anchoring to neuronal membranes4.Tetanus toxin spreads in the body through the lymphatic and vascular systems to the neuromuscular junction, internalized to peripheral neuron synapses, and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons to the CNS5. There, it inhibits neurotransmitter release by the specific degradation of the vesicle-secretory protein-Synaptobrevin6.

Molecular Weight: 150 kDa

UniProt: P04958