Taipoxin Protein

Catalog No. ABIN7582362

This Native Taipoxin protein is expressed in Snake (Serpentes).

Quick Overview for Taipoxin Protein (ABIN7582362)

Target: Taipoxin

Protein Type: Native

Origin: Oxyuranus scutellatus scutellatus

Source: Snake (Serpentes)

Application: Blocking Peptide (BP), Immunocytochemistry (ICC)

Purity: ≥97 % (HPLC)

Product Details

Purpose: A Blocker of Synaptic Vesicle Recycling

Sequence: i±, i² and i³ chains non-covalently linkedS

Specificity: Highly disulfide bridged protein, glycosylated

Characteristics: A Blocker of Synaptic Vesicle Recycling

Application Details

Application Notes: Antigen Preadsorption Control: 10 - 50 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from filtrated Ammonium acetate solution. May contain acetate as a residual counter ion.

Storage: 4 °C,-20 °C

Storage Comment: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Target Details

Target: Taipoxin

Background: Taipan toxin,Taipoxin is a lethal neurotoxin protein isolated from the venom of the Australian taipan Oxyuranus s. scutellatus (LD50= 2 μg/kg in mouse). Taipoxin is a non-covalent ternary glycoprotein which is completely dissociated at low pH or high ionic strength. One of the subunits has Phospholipase A2 activity1,2.Intoxicated animals die of asphyxia caused by a complete inhibition of neurotransmitter release and neuromuscular junction blockage of the respiratory muscles. In the central nervous system taipoxin blocks synaptic vesicle recycling by inhibiting the neuronal uptake pathway through the interaction with neuronal Pentraxin system components during synapse formation and remodeling1,3-5.In neuronal cultures, Taipoxin facilitates Ca2+-dependent synaptic vesicle exocytosis and causes a complete depletion of stored neurotransmitter, resulting in synaptic transmission blockage. Taipoxin, at nanomolar range, causes swelling of nerve terminus and redistribution of synaptic vesicle proteins7.

Molecular Weight: 46 kDa