ACVA Protein (AA 311-426) (Biotin,AVI tag)

Catalog No. ABIN7583221

Recombinant ACVA protein expressed in HEK-293 Cells.

Quick Overview for ACVA Protein (AA 311-426) (Biotin,AVI tag) (ABIN7583221)

Target: ACVA (Activin A (ACVA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 311-426

Purification tag / Conjugate: This ACVA protein is labelled with Biotin,AVI tag.

Purpose: Biotinylated Human Activin A Protein

Sequence: Gly311-Ser426

Characteristics: Recombinant Biotinylated Human Activin A Protein is expressed from HEK293 with Avi tag at the C-terminus., It contains Gly311-Ser426.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCL.

Buffer: Lyophilized from 0.22 μm filtered solution in 4 mM HCL. Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ACVA (Activin A (ACVA))

Alternative Name: Activin A

Background: Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.

Molecular Weight: 14.78 kDa. Due to glycosylation, the protein migrates to 15-18 kDa based on Tris-Bis PAGE result.

UniProt: P08476

Pathways: Hormone Transport, Peptide Hormone Metabolism