MSTN Protein (AA 24-375) (His tag)

Catalog No. ABIN7583273

This Recombinant MSTN protein is produced in HEK-293 Cells.

Quick Overview for MSTN Protein (AA 24-375) (His tag) (ABIN7583273)

Target: MSTN (Myostatin (MSTN))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-375

Purification tag / Conjugate: This MSTN protein is labelled with His tag.

Purpose: Human Latent GDF-8 Protein (pro&latent)

Sequence: Asn24-Ser375

Characteristics: Recombinant Human Latent GDF-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asn24-Ser375.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Latent GDF-8, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Apitegromab, hFc Tag with the EC50 of 21.5 ng/ml determined by ELISA.,Immobilized Human Latent GDF-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 33.7ng/ml determined by ELISA.,Human Latent GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Latent GDF-8 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MSTN (Myostatin (MSTN))

Alternative Name: Latent GDF-8

Background: Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.

Molecular Weight: 41.19 kDa. Due to glycosylation, the protein migrates to 14 kDa, 38-40 kDa and 45-52 kDa based on Bis-Tris PAGE result.

UniProt: O14793