MSTN Protein (AA 267-375)

Catalog No. ABIN7583284

Recombinant MSTN protein expressed in HEK-293 Cells.

Quick Overview for MSTN Protein (AA 267-375) (ABIN7583284)

Target: MSTN (Myostatin (MSTN))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse, Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 267-375

Purpose: Human/Mouse/Rat GDF-8 Protein

Sequence: Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat)

Characteristics: Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).,Biotinylated Activin RIIB, His Tag-captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).,Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).,Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.,Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.,Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA.,Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.,Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 4 mM HCL Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MSTN (Myostatin (MSTN))

Alternative Name: GDF-8

Background: Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.

Molecular Weight: 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.

UniProt: O14793