ACVR2A Protein (AA 20-135) (His tag)

Catalog No. ABIN7583285

This Recombinant ACVR2A protein is produced in HEK-293 Cells.

Quick Overview for ACVR2A Protein (AA 20-135) (His tag) (ABIN7583285)

Target: ACVR2A (Activin A Receptor, Type IIA (ACVR2A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-135

Purification tag / Conjugate: This ACVR2A protein is labelled with His tag.

Purpose: Mouse Activin RIIA/ACVR2A Protein

Sequence: Ala20-Pro135

Characteristics: Recombinant Mouse Activin RIIA/ACVR2A Protein is expressed from HEK293 with His tag at the C-terminus, It contains Ala20-Pro135.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Activin RIIA , His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 46.06 pM as determined in SPR assay (Biacore T200).,Mouse Activin RIIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Activin RIIA is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ACVR2A (Activin A Receptor, Type IIA (ACVR2A))

Alternative Name: Activin RIIA

Background: Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer.

Molecular Weight: 15.06 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

UniProt: P27038