IGFBPI Protein (AA 26-259)
Quick Overview for IGFBPI Protein (AA 26-259) (ABIN7677919)
Target
See all IGFBPI ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 26-259
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Sequence
- APWQCAPCSA EKLALCPPVS ASCSEVTRSA GCGCCPMCAL PLGAACGVAT ARCARGLSCR ALPGEQQPLH ALTRGQGACV QESDASAPHA AEAGSPESPE STEITEEELL DNFHLMAPSE EDHSILWDAI STYDGSKALH VTNIKKWKEP CRIELYRVVE SLAKAQETSG EEISKFYLPN CNKNGFYHSR QCETSMDGEA GLCWCVYPWN GKRIPGSPEI RGDPNCQIYF NVQN
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured by its ability to inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range ≤3 ug/ml.
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Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) protein (His tag)
IGFBPI Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) (AA 26-259) protein (His tag)IGFBPI Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) (AA 26-272) protein (His-IF2DI Tag)IGFBPI Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) (AA 1-259) protein (GST tag)IGFBPI Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) (AA 26-259) (Active) protein (His tag)IGFBPI Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
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Alternative Name
- IGFBP-1
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Background
- IGFBP-1(Insulin-like growth factor-binding protein 1), also known as PP12(placental protein 12), is a member of the Insulin-like growth factor-binding protein family. This protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. Recombinant human IGFBP-1 was expressed in HEK293 cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 25.2kDa (234aa)
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NCBI Accession
- NP_000587
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Pathways
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
Target
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