IGFBPI Protein (AA 26-259)

Catalog No. ABIN7596129

Recombinant IGFBPI protein expressed in HEK-293 Cells.

Quick Overview for IGFBPI Protein (AA 26-259) (ABIN7596129)

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 26-259

Sequence: APWQCAPCSA EKLALCPPVS ASCSEVTRSA GCGCCPMCAL PLGAACGVAT ARCARGLSCR ALPGEQQPLH ALTRGQGACV QESDASAPHA AEAGSPESPE STEITEEELL DNFHLMAPSE EDHSILWDAI STYDGSKALH VTNIKKWKEP CRIELYRVVE SLAKAQETSG EEISKFYLPN CNKNGFYHSR QCETSMDGEA GLCWCVYPWN GKRIPGSPEI RGDPNCQIYF NVQN

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured by its ability to inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range ≤3 ug/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Alternative Name: IGFBP-1

Background: IGFBP-1(Insulin-like growth factor-binding protein 1), also known as PP12(placental protein 12), is a member of the Insulin-like growth factor-binding protein family. This protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. Recombinant human IGFBP-1 was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Molecular Weight: 25.2kDa (234aa)

NCBI Accession: NP_000587

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding