DR3/LARD Protein (AA 25-199) (hIgG-His-tag)

Catalog No. ABIN7596180

Recombinant DR3/LARD protein expressed in Baculovirus infected Insect Cells.

Quick Overview for DR3/LARD Protein (AA 25-199) (hIgG-His-tag) (ABIN7596180)

Target: DR3/LARD (TNFRSF25) (Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 25-199

Purification tag / Conjugate: This DR3/LARD protein is labelled with hIgG-His-tag.

Sequence: QGGTRSP RCDCAGDFHK KIGLFCCRGC PAGHYLKAPC TEPCGNSTCL VCPQDTFLAW ENHHNSECAR CQACDEQASQ VALENCSAVA DTRCGCKPGW FVECQVSQCV SSSPFYCQPC LDCGALHRHT RLLCSRRDTD CGTCLPGFYE HGDGCVSCPT STLGSCPERC AAVCGWRQ

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured by its binding ability in a functional ELISA with Human TL1A/TNFSF15 (CAT# ATGP1049). The ED50 range ≤ 5 ug/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: DR3/LARD (TNFRSF25) (Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25))

Alternative Name: DR3/TNFRSF25

Background: TNFRSF25, also known as Tumor necrosis factor receptor superfamily member 25, is a member of the TNF receptor superfamily that binds to the TNF-like protein TL1A. TNFRSF25 signals are required to exert T helper cell 2 effector function in Th2-polarized CD4 cells and co-stimulate interleukin-13 production by glycosphingolipidactivated NKT cells. Its axis is a novel immune pathway that participates in the pathogenesis of a variety of autoimmune rheumatic diseases. These molecules may be promising therapeutic targets for inflammatory arthritis. Recombinant human TNFRSF25, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 46.1kDa (417aa)

NCBI Accession: NP_003781

Pathways: Apoptosis