DR3/LARD Protein (AA 25-199) (Fc Tag)

Catalog No. ABIN7092807

Recombinant DR3/LARD protein expressed in HEK-293 Cells.

Quick Overview for DR3/LARD Protein (AA 25-199) (Fc Tag) (ABIN7092807)

Target: DR3/LARD (TNFRSF25) (Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 25-199

Purification tag / Conjugate: This DR3/LARD protein is labelled with Fc Tag.

Purpose: Recombinant Human TNFRSF25 with C-terminal human Fc tag

Specificity: TNFRSF25(Gln25-Gln199) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: DR3/LARD (TNFRSF25) (Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25))

Alternative Name: TNFRSF25

Background: APO-3,DDR3,DR3,GEF720,LARD,PLEKHG5,TNFRSF12,TR3,TRAMP,WSL-1,WSL-LR
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 45.0 kDa after removal of the signal peptide. The apparent molecular mass of TNFRSF25-hFc is 55-70 kDa due to glycosylation.

UniProt: Q93038

Pathways: Apoptosis