CA10 Protein (AA 22-328) (His tag)
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- Target See all CA10 Proteins
- CA10 (Carbonic Anhydrase X (CA10))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 22-328
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CA10 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- Sequence
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QQNSPK IHEGWWAYKE VVQGSFVPVP SFWGLVNSAW NLCSVGKRQS PVNIETSHMI FDPFLTPLRI NTGGRKVSGT MYNTGRHVSL RLDKEHLVNI SGGPMTYSHR LEEIRLHFGS EDSQGSEHLL NGQAFSGEVQ LIHYNHELYT NVTEAAKSPN GLVVVSIFIK VSDSSNPFLN RMLNRDTITR ITYKNDAYLL QGLNIEELYP ETSSFITYDG SMTIPPCYET ASWIIMNKPV YITRMQMHSL RLLSQNQPSQ IFLSMSDNFR PVQPLNNRCI RTNINFSLQG KDCPNNRAQK LQYRVNEWLL K - Purity
- > 95% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 150 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH8.0 at 37°C.
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Carbonic Anhydrase X (CA10) (AA 1-328) protein (His tag)
custom-made CA10 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Carbonic Anhydrase X (CA10) (AA 1-328) protein (His tag)custom-made CA10 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Carbonic Anhydrase X (CA10) (Active) protein (His tag)CA10 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- CA10 (Carbonic Anhydrase X (CA10))
- Alternative Name
- Carbonic Anhydrase X/CA10 (CA10 Products)
- Background
- Carbonic anhydrase X, also known as CA10, belongs to the CA family of zinc metalloenzymes. It is catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. Also an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development. Recombinant human Carbonic Anhydrase X/CA10, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.3kDa (317aa)
- NCBI Accession
- NP_064563
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