Asparagine Synthetase Protein (ASNS) (AA 1-561) (His tag)

Catalog No. ABIN7677055

This Recombinant Asparagine Synthetase protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for Asparagine Synthetase Protein (ASNS) (AA 1-561) (His tag) (ABIN7677055)

Target: Asparagine Synthetase (ASNS)

Protein Type: Recombinant

Origin: Mouse

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-561

Purification tag / Conjugate: This Asparagine Synthetase protein is labelled with His tag.

Sequence: MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL AVVDPLFGMQ PIRVRKYPYL WLCYNGEIYN HKALQQRFEF EYQTNVDGEI ILHLYDKGGI EKTICMLDGV FAFILLDTAN KKVFLGRDTY GVRPLFKAMT EDGFLAVCSE AKGLVSLKHS TTPFLKVEPF LPGHYEVLDL KPNGKVASVE MVKYHHCTDE PLHAIYDSVE KLFPGFDLET VKNNLRILFD NAIKKRLMTD RRIGCLLSGG LDSSLVAASL LKQLKEAQVQ YPLQTFAIGM EDSPDLLAAR KVANYIGSEH HEVLFNSEEG IQALDEVIFS LETYDITTVR ASVGMYLISK YIRKNTDSVV IFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLKELYLF DVLRADRTTA AHGLELRVPF LDHRFSSYYL SLPPDMRIPK NGIEKHLLRE TFEDCNLLPK EILWRPKEAF SDGITSVKNS WFKILQDYVE HQVDDEMMSA SQKFPFNTP KTKEGYFYRQ IFERHYPGRA DWLTHYWMPK WINATDPSAR TLTHYKSAAK A

Purification: > 95% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 40 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Asparagine Synthetase (ASNS)

Alternative Name: Asparagine Synthetase/ASNS

Background: ASNS, also known as Asparagine synthetase, is a one of cytoplasmic enzyme that generates asparagine from aspartate. It mainly distributes in mammalian organs, but basal expression is low in tissues other than the exocrine pancreas. This protein plays a role of cell growth, and its mRNA content is linked to changes in the cell cycle. Its mRNA is upregulated in response to the asparagine depletion, some of the toxic effects of themedication on normal cell activity. Above average presence of asparagine synthetase in certain leukemia strains has been linked to be a significant contributing factor of chemotherapy resistance, particularly to thechemotherapy drug, L-asparaginase. Also, it functions as potential biomarker for ovarian cancer, potential role in solid tumor metastasis. Recombinant mouse ASNS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 65.1 kDa (567aa)

NCBI Accession: NP_036185

Pathways: ER-Nucleus Signaling