DC-SIGN/CD209 Protein (AA 60-404) (hIgG-His-tag)

Catalog No. ABIN7596355

Product Details

Target: DC-SIGN/CD209 (CD209) (CD209)

Protein Type: Recombinant

Protein Characteristics: AA 60-404

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This DC-SIGN/CD209 protein is labelled with hIgG-His-tag.

Application: SDS-PAGE (SDS)

Sequence: VSKVPSS ISQEQSRQDA IYQNLTQLKA AVGELSEKSK LQEIYQELTQ LKAAVGELPE KSKLQEIYQE LTRLKAAVGE LPEKSKLQEI YQELTWLKAA VGELPEKSKM QEIYQELTRL KAAVGELPEK SKQQEIYQEL TRLKAAVGEL PEKSKQQEIY QELTRLKAAV GELPEKSKQQ EIYQELTQLK AAVERLCHPC PWEWTFFQGN CYFMSNSQRN WHDSITACKE VGAQLVVIKS AEEQNFLQLQ SSRSNRFTWM GLSDLNQEGT WQWVDGSPLL PSFKQYWNRG EPNNVGEEDC AEFSGNGWND DKCNLAKFWI CKKSAASCSR DEEQFLSPAP ATPNPPPA

Purity: > 90% by SDS - PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: DC-SIGN/CD209 (CD209 Products)

Background: DC-SIGN/CD209, also known as Dendritic Cell-specific ICAM-3 Grabbing Non-integrin, is a member of the C-type lectin family. It is Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. DC-SIGN on macrophages recognises and binds with high affinity to high-mannose type N-glycans, a class of pathogen associated molecular patterns PAMPs commonly found on viruses, bacteria and fungi. This binding interaction activates phagocytosis. It has a high affinity for the ICAM3 Molecule and binds various microorganisms by recognizing high-mannose-containing glycoproteins on their envelopes and especially functions as receptor for several viruses such as HIV and Hepatitis C. Besides functioning as an adhesion molecule, recent study has also shown that DC-SIGN can initiate innate immunity by modulating toll-like receptors, though the detailed mechanism is not yet known. It together with other C-type lectins is involved in recognition of tumors by dendritic cells. It is also a potential engineering target for dendritic cell based cancer vaccine. Recombinant human DC-SIGN/CD209, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 66.5kDa (587aa)

NCBI Accession: NP_066978