SUMF1 Protein (AA 34-374) (His tag)
-
- Target See all SUMF1 Proteins
- SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 34-374
-
Origin
- Human
-
Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This SUMF1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
-
SQEAGTGAGA GSLAGSCGCG TPQRPGAHGS SAAAHRYSRE ANAPGPVPGE RQLAHSKMVP IPAGVFTMGT DDPQIKQDGE APARRVTIDA FYMDAYEVSN TEFEKFVNST GYLTEAEKFG DSFVFEGMLS EQVKTNIQQA VAAAPWWLPV KGANWRHPEG PDSTILHRPD HPVLHVSWND AVAYCTWAGK RLPTEAEWEY SCRGGLHNRL FPWGNKLQPK GQHYANIWQG EFPVTNTGED GFQGTAPVDA FPPNGYGLYN IVGNAWEWTS DWWTVHHSVE ETLNPKGPPS GKDRVKKGGS YMCHRSYCYR YRCAARSQNT PDSSASNLGF RCAADRLPTM D
- Purity
- > 85% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product SUMF1 Protein
-
Sulfatase Modifying Factor 1 (SUMF1) (AA 101-216) protein (His-IF2DI Tag)
SUMF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Sulfatase Modifying Factor 1 (SUMF1) (AA 91-374) protein (His tag)SUMF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85% by SDS-PAGE SDS
Sulfatase Modifying Factor 1 (SUMF1) (AA 89-188) protein (GST tag)SUMF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sulfatase Modifying Factor 1 (SUMF1) proteinSUMF1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Sulfatase Modifying Factor 1 (SUMF1) protein (Myc-DYKDDDDK Tag)SUMF1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
-
- Target
- SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))
- Alternative Name
- Sulfatase Modifying Factor 1/SUMF1 (SUMF1 Products)
- Background
- SUMF1, also known as sulfatase-modifying factor 1 isoform 1, is a Ca2+-binging member of the sulfatase-modifying factor family. This protein as soluble ER lumenal glycoprotein converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, it can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in inactive FGE, and subsequently multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant Human SUMF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 38.1 kDa (347aa)
- NCBI Accession
- NP_877437
-
