Peropsin Protein (RRH) (DYKDDDDK Tag,Strep Tag)

Catalog No. ABIN7596932

This Synthetic Nanodisc Peropsin protein is expressed in HEK-293 Cells.

Quick Overview for Peropsin Protein (RRH) (DYKDDDDK Tag,Strep Tag) (ABIN7596932)

Target: Peropsin (RRH)

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Application: ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Product Details

Purification tag / Conjugate: This Peropsin protein is labelled with DYKDDDDK Tag,Strep Tag.

Purpose: Human RRH-Strep full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Handling Advice: Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Peropsin (RRH)

Alternative Name: RRH

Background: N/A
Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. May play a role in rpe physiology either by detecting light directly or by monitoring the concentration of retinoids or other photoreceptor-derived compounds.

Molecular Weight: The human full length RRH-Strep protein has a MW of 37.4 kDa

UniProt: O14718