TAS2R1 Protein (DYKDDDDK Tag,Strep Tag)

Catalog No. ABIN7596951

Synthetic Nanodisc TAS2R1 protein expressed in HEK-293 Cells.

Quick Overview for TAS2R1 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7596951)

Target: TAS2R1 (Taste Receptor, Type 2, Member 1 (TAS2R1))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Application: ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Product Details

Purification tag / Conjugate: This TAS2R1 protein is labelled with DYKDDDDK Tag,Strep Tag.

Purpose: Human TA2R1-Strep full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: TAS2R1 (Taste Receptor, Type 2, Member 1 (TAS2R1))

Alternative Name: TA2R1

Background: T2R1, TRB7
This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is mapped to chromosome 5p15, the location of a genetic locus (PROP) that controls the detection of the bitter compound 6-n-propyl-2-thiouracil. [provided by RefSeq, Jul 2008]

Molecular Weight: The human full length TA2R1-Strep protein has a MW of 34.3 kDa

UniProt: Q9NYW7