CACNA2D1 Protein (DYKDDDDK Tag,Strep Tag)

Catalog No. ABIN7854954

Synthetic Nanodisc CACNA2D1 protein expressed in HEK-293 Cells.

Quick Overview for CACNA2D1 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7854954)

Target: CACNA2D1 (Calcium Channel, Voltage-Dependent, alpha 2/delta Subunit 1 (CACNA2D1))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Application: ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Product Details

Purification tag / Conjugate: This CACNA2D1 protein is labelled with DYKDDDDK Tag,Strep Tag.

Purpose: Human CA2D1-Strep full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CACNA2D1 (Calcium Channel, Voltage-Dependent, alpha 2/delta Subunit 1 (CACNA2D1))

Alternative Name: CA2D1

Background: CACNA2, CACNL2A, CCHL2A, LINC01112, lncRNA-N3
The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion. [provided by RefSeq, Nov 2014]

Molecular Weight: The human full length CA2D1-Strep protein has a MW of 124.6 kDa

UniProt: P54289