MLC1 Protein (DYKDDDDK Tag)

Catalog No. ABIN7597220

This MNP Membrane Nanoparticle MLC1 protein is expressed in HEK-293 Cells.

Quick Overview for MLC1 Protein (DYKDDDDK Tag) (ABIN7597220)

Target: MLC1

Protein Type: MNP Membrane Nanoparticle

Origin: Human

Source: HEK-293 Cells

Product Details

Purification tag / Conjugate: This MLC1 protein is labelled with DYKDDDDK Tag.

Purpose: Human MLC1 full length protein-MNP

Characteristics: Plasma membrane-coated nanoparticles (MNPs) have been used in various applications, including delivery of therapeutic agents and induction of immune responses et al. Unlike the conventional strategies, MNPs directly leverage intact and natural functions of cell membranes, and show high biocompatibility, specificity, and low side effects. Our optimized MNPs platform for the full-length membrane protein production uses membrane coating technology and a HEK293 based expression platform. The high-purity plasma membrane-coated nanoparticles were produced by extrusion after membrane extraction from the host HEK293 cells containing the overexpressed target proteins.

Application Details

Comment:

Advantages of MNP:

• High display density of target membrane proteins
• Native structure and orientation of transmembrane protein
• soluble in aqueous solutions for routine biochemical analysis
• Detergent-free purification process
• Strong immunogenicity
• Works for MPs that can´t be produced via VLPs and EXos

Limitations of MNP:

• Lack of accurate quantification of the target membrane proteins.
• Need to develop special SPR assayx.
• Cell membranes contain housekeeping proteins that can result in immune response dilution.
• Some membrane proteins can´t be enriched on membrane.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from PBS. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: MLC1

Background: LVM, MLC, VL
The function of this protein is unknown, however, homology to other proteins suggests that it may be an integral membrane transporter. Mutations in this gene have been associated with megalencephalic leukoencephalopathy with subcortical cysts, an autosomal recessive neurological disorder. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: The human full length MLC1 protein has a MW of 41.2 kDa

UniProt: Q15049