CA9 Protein (AA 38-137) (Fc Tag)

Catalog No. ABIN7597392

Recombinant CA9 protein expressed in HEK-293 Cells.

Quick Overview for CA9 Protein (AA 38-137) (Fc Tag) (ABIN7597392)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 38-137

Purification tag / Conjugate: This CA9 protein is labelled with Fc Tag.

Purpose: Recombinant human CA9(38-137) Protein with C-terminal human Fc tag

Sequence: CA9(Gln38-Asp137) hFc(Glu99-Ala330)

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: CA9

Background: MN, CAIX
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Molecular Weight: predicted molecular mass of 37.0 kDa after removal of the signal peptide. The apparent molecular mass of CA9(38-137)-hFc is 35-55 kDa due to glycosylation.

UniProt: Q16790