CA12 Protein (AA 25-301) (His tag)

Catalog No. ABIN7597420

This Recombinant CA12 protein is produced in HEK-293 Cells.

Quick Overview for CA12 Protein (AA 25-301) (His tag) (ABIN7597420)

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 25-301

Purification tag / Conjugate: This CA12 protein is labelled with His tag.

Purpose: Recombinant human CA12 Protein with C-terminal 10xHis tag

Sequence: CA12(Ala25-Ser301) 10xHis tag

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Alternative Name: CA12

Background: CAXII, CA-XII, T18816, HsT18816
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10 % of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]

Molecular Weight: predicted molecular mass of 32.5 kDa after removal of the signal peptide. The apparent molecular mass of CA12-His is 25-55 kDa due to glycosylation.

UniProt: O43570