GPBAR1 Protein (AA 1-19) (Fc Tag)

Catalog No. ABIN7597436

This Recombinant GPBAR1 protein is expressed in HEK-293 Cells.

Quick Overview for GPBAR1 Protein (AA 1-19) (Fc Tag) (ABIN7597436)

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 1-19

Purification tag / Conjugate: This GPBAR1 protein is labelled with Fc Tag.

Purpose: Recombinant human GPBAR1 Protein with C-terminal human Fc tag

Sequence: GPBAR1(Met1-Gly19) hFc(Glu99-Ala330)

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Alternative Name: GPBAR1

Background: BG37, TGR5, M-BAR, GPCR19, GPR131
This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 28.0 kDa after removal of the signal peptide. The apparent molecular mass of GPBAR1-hFc is 25-55 kDa due to glycosylation.

UniProt: Q8TDU6

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound