SPARC Protein (AA 18-303) (His tag)
Quick Overview for SPARC Protein (AA 18-303) (His tag) (ABIN7597443)
Target
See all SPARC ProteinsProtein Type
Origin
Source
Application
Purity
-
-
Protein Characteristics
- AA 18-303
-
Purification tag / Conjugate
- This SPARC protein is labelled with His tag.
-
Purpose
- Recombinant human SPARC Protein with C-terminal 10xHis tag
-
Specificity
- SPARC(Ala18-Ile303) 10xHis tag
-
Characteristics
- Extracellular Domain Protein
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!Your project requires further customization? Contact us and discover our custom protein solutions
-
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) (Active) protein (His tag)
SPARC Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) protein (His tag)SPARC Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) protein (GST tag)SPARC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-297) protein (His-IF2DI Tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 99-330) protein (Fc Tag)SPARC Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
-
-
Application Notes
-
Extracellular Domain Proteins (ECD) can be used as:
- Immunogens for antibody drug development
- Reagents used for CAR-T positive cell monitoring
- Reagents for antibody screening and functional testing
- Reagents for antibody affinity measurement
-
Comment
-
The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- Please see Certificate of Analysis for specific instructions of reconstitution.
-
Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
-
Storage
- -20 °C,-80 °C
-
Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
-
Expiry Date
- 12 months
-
-
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
-
Alternative Name
- SPARC
-
Background
-
ON, ONT, OI17, BM-40
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015] -
Molecular Weight
- predicted molecular mass of 34.1 kDa after removal of the signal peptide. The apparent molecular mass of SPARC-His is 35-55 kDa due to glycosylation.
-
UniProt
- P09486
-
Pathways
- Autophagy
Target
-
