Klotho beta Protein (KLB) (AA 35-982) (His tag)

Catalog No. ABIN7607182

This Recombinant Klotho beta protein is produced in Mammalian Cells.

Quick Overview for Klotho beta Protein (KLB) (AA 35-982) (His tag) (ABIN7607182)

Target: Klotho beta (KLB)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 35-982

Purification tag / Conjugate: This Klotho beta protein is labelled with His tag.

Purpose: Recombinant Mouse KLB/Beta-klotho Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using mouse pro-B cells (BaF3) transfected with human FGF RIIIc. The ED50 for this effect is 0.04-0.4 μg/mL in the presence of 1 μg/mL of recombinant human FGF23 and 10 μg/mL of heparin.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: Klotho beta (KLB)

Alternative Name: Beta-klotho

Background: Beta-klotho, BKL, BetaKlotho, Klotho beta-like protein, KLB

UniProt: O35082

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Growth Factor Binding