CHI3L3 Protein (AA 1-398) (His tag)

Catalog No. ABIN7678260

This Recombinant CHI3L3 protein is produced in Mammalian Cells.

Quick Overview for CHI3L3 Protein (AA 1-398) (His tag) (ABIN7678260)

Target: CHI3L3 (Chitinase 3-Like 3 (CHI3L3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Mammalian Cells

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Activity Assay (AcA), Immunogen (Imm)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-398

Purification tag / Conjugate: This CHI3L3 protein is labelled with His tag.

Purpose: Recombinant Mouse Chil3 Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of human squamous cell carcinoma cells (FaDu). When 5 x 10^4 cells/well are added to recombinant Mouse Chil3/Ym1 (ABIN7607185 and ABIN7678260) coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.3-1.2 μg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: CHI3L3 (Chitinase 3-Like 3 (CHI3L3))

Alternative Name: Chitinase-like protein 3

Background: Chitinase-like protein 3, Beta-N-acetylhexosaminidase Ym1, Chitinase-3-like protein 3, ECF-L, Eosinophil chemotactic cytokine, Secreted protein Ym1, Chil3, Chi3l3, Ym1

UniProt: O35744