PEMT Protein (AA 1-167) (His-Fc tag)

Catalog No. ABIN7678403

Recombinant PEMT protein expressed in Mammalian Cells.

Quick Overview for PEMT Protein (AA 1-167) (His-Fc tag) (ABIN7678403)

Target: PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: ELISA, Western Blotting (WB), Activity Assay (AcA), Immunogen (Imm), SDS-PAGE (SDS)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-167

Purification tag / Conjugate: This PEMT protein is labelled with His-Fc tag.

Purpose: Recombinant Human CD227/MUC1 Protein, C-hFc-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to increase beta-catenin levels in the cytoplasm and nucleus of human colon adenocarcinoma cells (HCT116). 0.01-1 ng/mL of recombinant human CD227/MUC1 (ABIN7607340 and ABIN7678403) can effectively increase beta-catenin levels.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))

Alternative Name: PEMT

Background: PEMT, CA 15-3, H23AG, Peanut-reactive urinary mucin, PEM, EMA, Cancer antigen 15-3, MUC-1, Episialin, MUC1-CT, MUC1-beta, Tumor-associated mucin, CD227, Polymorphic epithelial mucin, KL-6, MUC1-alpha, MUC1, PUM, Krebs von den Lungen-6, MUC1-NT, Tumor-associated epithelial membrane antigen, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin

UniProt: P15941