MCAM Protein (AA 24-559) (His tag)

Catalog No. ABIN7607458

This Recombinant MCAM protein is produced in Mammalian Cells.

Quick Overview for MCAM Protein (AA 24-559) (His tag) (ABIN7607458)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 24-559

Purification tag / Conjugate: This MCAM protein is labelled with His tag.

Purpose: Recombinant Human CD146/MCAM Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. When rh CD146/MCAM (Cat #: AHE39701) is immobilized at 1 μg/mL, 100 μL/well, it binds recombinant human Galectin-3. The concentration of recombinant human Galectin-3 that produces 50% of the optimal binding response is 0.25-1.25 μg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12

UniProt: P43121