LUM Protein (AA 19-338) (His tag)

Catalog No. ABIN7607480

This Recombinant LUM protein is expressed in Mammalian Cells.

Quick Overview for LUM Protein (AA 19-338) (His tag) (ABIN7607480)

Target: LUM (Lumican (LUM))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Immunogen (Imm), Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 19-338

Purification tag / Conjugate: This LUM protein is labelled with His tag.

Purpose: Recombinant Mouse LUM/Lumican Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of bovine corneal endothelial cells (BCE C/D-1b). When 5 x 10^4 cells per well are added to rmLUM/Lumican (Cat #: AME89701) coated plates (10 μg/mL, 100 μL/well) ≥ 50% will adhere after 50 minutes at 37 °C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: LUM (Lumican (LUM))

Alternative Name: Keratan sulfate proteoglycan lumican

Background: Keratan sulfate proteoglycan lumican, Lumican, SLRR2D, LDC, LUM, KSPG lumican

UniProt: P51885

Pathways: Glycosaminoglycan Metabolic Process