Protein A Protein (His tag)

Catalog No. ABIN7665109

This Recombinant Protein A protein is expressed in Escherichia coli (E. coli).

Quick Overview for Protein A Protein (His tag) (ABIN7665109)

Target: Protein A

Protein Type: Recombinant

Origin: Staphylococcus aureus

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This Protein A protein is labelled with His tag.

Purpose: Recombinant ProA-B4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Protein A

Background: Protein A

Molecular Weight: 28.3 kDa

UniProt: P38507