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Protein A Protein (Biotin)

Origin: Staphylococcus aureus ELISA, WB, DB, IHC
Catalog No. ABIN1536532
  • Target See all Protein A Proteins
    Protein A
    Origin
    Staphylococcus aureus
    Host
    Please inquire
    Purification tag / Conjugate
    This Protein A protein is labelled with Biotin.
    Application
    ELISA, Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC)
  • Application Notes
    Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:5,000-1:50,000 Western blot: 1:5,000-1:50,000 ELISA: 1:50,000-1:500,000 Other: user-optimized.
    Restrictions
    For Research Use only
  • Concentration
    2 mg/ml
    Buffer
    PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use.
  • Wen, He, Lee: "Specific antibody immobilization with biotin-poly(L-lysine)-g-poly(ethylene glycol) and protein A on microfluidic chips." in: Journal of immunological methods, Vol. 350, Issue 1-2, pp. 97-105, (2009) (PubMed).

  • Target
    Protein A
    Abstract
    Protein A Products
    Synonyms
    alpha protein Protein, repB Protein
    Background
    Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. The product contains five high-affinity binding sites capable of interacting with the Fc region of IgG in humans, rabbits, pigs, and other species. Biotin-Protein A, which is produced by covalently linking biotin to protein A, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
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