Vinculin Protein (VCL) (AA 2-1066)

Catalog No. ABIN7666017

This Recombinant Vinculin protein is produced in Escherichia coli (E. coli).

Quick Overview for Vinculin Protein (VCL) (AA 2-1066) (ABIN7666017)

Target: Vinculin (VCL)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 2-1066

Purpose: Recombinant Human Vinculin

Endotoxin Level: < 1 EU/μg as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20°C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80°C for 3 months or 1 week at 2 to 8°C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: Vinculin, Metavinculin, VCL

Molecular Weight: 115 kDa

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2