Adrenomedullin Protein (ADM) (His tag)

Catalog No. ABIN7668126

Recombinant Adrenomedullin protein expressed in Escherichia coli (E. coli).

Quick Overview for Adrenomedullin Protein (ADM) (His tag) (ABIN7668126)

Target: Adrenomedullin (ADM)

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This Adrenomedullin protein is labelled with His tag.

Purpose: Recombinant Rat Adrenomedullin/ADM protein (His tag)

Sequence: Thr 21-Leu 185

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Adrenomedullin (ADM)

Alternative Name: Adrenomedullin,ADM

Target Type: Hormone

Background: Adrenomedullin (ADM, also AM) is a secreted, monomeric, 6 kDa member of the Adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. ADM has multiple functions, including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The ADM preproprecursor is 185 amino acids (aa) in length. It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-91), the ADM precursor (amidation is required for maturation) (aa 94 143), and a C-terminal propeptide (aa 150-185). The ADM precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated ADM (aa 94 143).,ADM precursor,Adrenomedullin,PAMP,Preproadrenomedullin,ProAM N terminal 20 peptide,ProAM N20

Molecular Weight:

calculated_mw: 18.0 kDa

observed_mw: 25 kDa

UniProt: P43145

Pathways: Hormone Transport, Hormone Activity, C21-Steroid Hormone Metabolic Process, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Tube Formation