HFM1 Protein (AA 5325-5925) (His-MBP)

Catalog No. ABIN7668895

This Recombinant HFM1 protein is produced in Escherichia coli (E. coli).

Quick Overview for HFM1 Protein (AA 5325-5925) (His-MBP) (ABIN7668895)

Target: HFM1 (HFM1, ATP-Dependent DNA Helicase Homolog (HFM1))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purity: > 80 %

Product Details

Protein Characteristics: AA 5325-5925

Purification tag / Conjugate: This HFM1 protein is labelled with His-MBP.

Purpose: Recombinant SARS-CoV-2 Helicase Protein (His Tag)

Sequence: Ala5325-Gln5925

Purification: > 80 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: HFM1 (HFM1, ATP-Dependent DNA Helicase Homolog (HFM1))

Alternative Name: Helicase

Background: The non-structural protein 13 (nsp13) of SARS-CoV 2 is a helicase that separates double-stranded RNA or DNA with a 5'-3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5'-3' direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom's syndrome, Werner's syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.,SARS-CoV 2 Helicase,SARS-CoV 2 nsp13

Molecular Weight:

calculated_mw: 112.8 kDa

observed_mw: 120 kDa