HSA Protein (AA 25-609)

Catalog No. ABIN7668910

This Recombinant HSA protein is produced in Yeast (Pichia pastoris).

Quick Overview for HSA Protein (AA 25-609) (ABIN7668910)

Target: HSA (Human Serum Albumin (HSA))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human

Source: Yeast (Pichia pastoris)

Purity: > 95 %

Product Details

Protein Characteristics: AA 25-609

Purpose: Recombinant Human Serum Albumin/HSA Protein (His Tag)

Sequence: Asp25-Leu609

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: HSA (Human Serum Albumin (HSA))

Alternative Name: Serum Albumin,HSA

Background: Human serum albumin (HSA), the most abundant protein in human blood plasma, is essential for maintaining osmotic pressure. It is produced in the liver, consists of a single polypeptide chain, with 585 amino acids with 17 tyrosil residues and one tryptophan located in position 214. HSA is organized in three domains, I, II and III, each consisting of two subdomains, A and B. In the physiological states, HSA occurs in two forms - the non-modified HSA with a free thiol group of Cys-34, and the modified (oxidized) form (oHSA), also called human mercaptoalbumin (HMA) and human nonmercaptoalbumin (HNA), respectively. HMA and HNA are in equilibrium, depending on the redox state of Cys-34, and their ratio also varies depending on age and the diseased state. HSA functions primarily as a carrier protein for drugs, steroids, fatty acids, and thyroid hormones, and plays a role in stabilizing extracellular fluid volume.,ALB,HAS,Serum Albumin

Molecular Weight:

calculated_mw: 66.5 kDa

observed_mw: 55-80 kDa

UniProt: P02768