HYAL1 Protein (AA 1-435) (His tag)

Catalog No. ABIN7668911

This Recombinant HYAL1 protein is expressed in Mammalian Cells.

Quick Overview for HYAL1 Protein (AA 1-435) (His tag) (ABIN7668911)

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human

Source: Mammalian Cells

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-435

Purification tag / Conjugate: This HYAL1 protein is labelled with His tag.

Purpose: Recombinant Human HYAL1 Protein(His Tag)

Sequence: Met1-Trp435

Purification: SDS-PAGE analysis of Human HYAL1 proteins, 2μg/lane of Recombinant Human HYAL1 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 50-60 kDa

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Alternative Name: HYAL1

Background: Human hyaluronidases (HYALs) are a group of five endo- beta -N-acetyl-hexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20). While HYAL1, HYAL2 and SPAM1 are endo- beta -N-acetyl-glucosaminidase that are mainly active on hyaluronan with little activity on chondroitin sulfate, HYAL4 is mainly active on chondroitin sulfate (type C and D in particular) (6). Hyaluronan and chondroitin sulfate are abundant extracellular matrix components that have numerous biological functions. The backbone of chondroitin sulfate composed of repeating units of -4GlcA beta 1-3GalNAc beta 1- most closely resembles to that of hyaluronan composed of repeating units of -4GlcA beta 1-3GlcNAc beta 1-, which may partially explain the overlapping substrate specificity of most hyaluronidases. HYAL1 is a lysosomal hyaluronidase with optimal activity around pH 4.0. It is highly expressed in the liver, kidney and heart, and is the predominant hyaluronidase found in plasma. Defects in HYAL1 are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. Surprisingly, HYAL1 has been reported both as a tumor promoter and suppressor. The enzymatic activity of recombinant human HYAL1 was measured using 35S-labeled hyaluronan as substrate.,NAT,MPS,Lung carcinoma protein,HYAL1,HYAL-1,LUCA1,MPS9,NAT6,Hyaluronic Lase,Hyaluronidase-1,Hyaluronoglucosaminidase-1,LuCa-1,HYAL 1,Hyaluronidase 1,hyaluronoglucosaminidase 1,LuCa 1,Lung carcinoma protein 1

Molecular Weight:

calculated_mw: 47.7 kDa

observed_mw: 50-60 kDa

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process