IL-25 Protein (His tag)

Catalog No. ABIN7669095

Recombinant IL-25 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-25 Protein (His tag) (ABIN7669095)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 98 %

Product Details

Purification tag / Conjugate: This IL-25 protein is labelled with His tag.

Purpose: Recombinant Human IL-25 protein(His Tag)

Sequence: Tyr 33-Gly 177

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <5 ng/mL.
2. Measure by its ability to induce CXCL1 secretion in HT29 cells. The ED₅₀ for this effect is <1 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL-25

Background: Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.,UNQ;Interleukin;IL-17E;IL-25;Interleukin-17E;Interleukin-25;IL17E;IL25;UNQ3120;PRO10272

Molecular Weight:

calculated_mw: 17.7 kDa

observed_mw: 19 kDa

UniProt: Q9H293