LTA Protein (His tag)

Catalog No. ABIN7669249

This Recombinant LTA protein is produced in Escherichia coli (E. coli).

Quick Overview for LTA Protein (His tag) (ABIN7669249)

Target: LTA (Lymphotoxin-alpha (LTA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 98 %

Product Details

Purification tag / Conjugate: This LTA protein is labelled with His tag.

Purpose: Recombinant Mouse TNF beta protein(His Tag)

Sequence: Leu 34-Leu 202

Purification: > 98 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce cytotoxicity in L929 cells in the presence of vactinomycin D. The ED₅₀ for this effect is <30 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: TNF beta

Background: Tumor Necrosis Factor β (TNF-β) is a secreted protein belonging to the tumor necrosis factor family. TNF-β binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM in homotrimeric form, binds to TNFRSF3/LTBR in heterotrimeric form with LTB. TNF-β forms heterotrimers with lymphotoxin-beta, which anchors TNF-β to the cell surface. TNF-β mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-β is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo.,IFL,IFL-1,IFN-λ3,INF-alpha,INF-l,INF-lambda

Molecular Weight:

calculated_mw: 19.4 kDa

observed_mw: 11-17 kDa

UniProt: P09225

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process