Merlin Protein (AA 1-595) (His tag)

Catalog No. ABIN7669357

This Recombinant Merlin protein is produced in Escherichia coli (E. coli).

Quick Overview for Merlin Protein (AA 1-595) (His tag) (ABIN7669357)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-595

Purification tag / Conjugate: This Merlin protein is labelled with His tag.

Purpose: Recombinant Human Merlin/NF2 protein (His Tag)

Sequence: Met1-Leu595

Purification: > 90 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % Tween 80.
Normally 5 %-8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Alternative Name: Merlin,NF2

Background: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.,BANF,Bilateral acoustic neuroma,CAN,MERL_HUMAN,Merlin,Moesin ezrin radixin like protein,Moesin ezrin radizin like,Moesin-ezrin-radixin-like,NF 2,Neurofibromatosis 2,Neurofibromatosis type 2,Neurofibromatosis2,SCH,Schwannomerlin

Molecular Weight:

calculated_mw: 69.7 kDa

observed_mw: 68-71 kDa

UniProt: P35240

Pathways: Cell-Cell Junction Organization