PODXL Protein (SUMO-His Tag)

Catalog No. ABIN7669448

This Recombinant PODXL protein is expressed in Escherichia coli (E. coli).

Quick Overview for PODXL Protein (SUMO-His Tag) (ABIN7669448)

Target: PODXL (Podocalyxin-Like (PODXL))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This PODXL protein is labelled with SUMO-His Tag.

Purpose: Recombinant Human PODXL protein (SUMO,His tag)

Sequence: Ala 220-Ile 431

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: PODXL

Background: Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family.Podocalyxin is expressed on glomerular and vascular endothelial cells, neurons, hematopoietic, mesenchymal, and cardiac stem cells, and metastatic carcinoma and glioblastoma tumor cells. A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia. Podocalyxin promotes platelet activation, neurite outgrowth, branching, and synaptogenesis, homing of hematopoietic progenitor cells to the bone marrow and spleen, and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney. Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium.,PCLP,PCLP-1,PCLP1,PCLPGCTM-2 antigen,PCpodocalyxin,POD XL,PODXL,Podocalyxin,Podocalyxin-like protein 1,podocalyxin-like

Molecular Weight:

calculated_mw: 34.2 kDa

observed_mw: 32 kDa

UniProt: O00592

Pathways: Tube Formation