SARS-CoV-2 NSP13 Protein (AA 5325-5925) (His tag)

Catalog No. ABIN7669571

This Recombinant SARS-CoV-2 NSP13 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SARS-CoV-2 NSP13 Protein (AA 5325-5925) (His tag) (ABIN7669571)

Target: SARS-CoV-2 NSP13 (HEL) (SARS-CoV-2 Helicase (NSP13) (HEL))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purity: > 90 %

Product Details

Protein Characteristics: AA 5325-5925

Purification tag / Conjugate: This SARS-CoV-2 NSP13 protein is labelled with His tag.

Purpose: Recombinant SARS-CoV-2 NSP13 protein

Sequence: Ala5325-Gln5925

Purification: > 90 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Application Details

Restrictions: For Research Use only

Handling

Buffer: Lyopilized from PBS pH 7. 4,0.02 % NLS, 1 mM EDTA, 4 %trehalose,1 % mannitol.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Other preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 NSP13 (HEL) (SARS-CoV-2 Helicase (NSP13) (HEL))

Alternative Name: SARS-CoV-2 NSP13

Target Type: Viral Protein

Background: NSP13 is a 67 kDa protein that belongs to the helicase superfamily 1B, it utilizes the energy of nucleotide triphosphate hydrolysis to catalyze the unwinding of double-stranded DNA or RNA in a 5' to 3' direction. Although NSP13 is believed to act on RNA in vivo enzymatic characterization shows a significantly more robust activity on DNA in in vitro assays with relatively weak non processive helicase activity when compared to other superfamily 1B enzymes. NSP13 has been shown to interact with the viral RNA-dependent RNA polymerase NSP1210,11, and acts in concert with the replication-transcription complex (NSP7/NSP8/NSP12). This interaction has been found to significantly stimulate the helicase activity of NSP13 possibly by means of mechano-regulation. In addition to its helicase activity, NSP13 also possesses RNA 5' triphosphatase activity within the same active site, suggesting a further essential role for NSP13 in the formation of the viral 5' mRNA cap.,Helicase,SARS-CoV 2 nsp13

Molecular Weight: 69.2 kDa

UniProt: P0DTC2