VWF Protein (His tag)

Catalog No. ABIN7669967

This Recombinant VWF protein is expressed in Escherichia coli (E. coli).

Quick Overview for VWF Protein (His tag) (ABIN7669967)

Target: VWF (Von Willebrand Factor (VWF))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This VWF protein is labelled with His tag.

Purpose: Recombinant Mouse VWF protein (His tag)

Sequence: Asp 1498-Val 1665

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: VWF

Background: Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.,F8VWF,VWD,VWF,Von Willebrand Factor,Vwf

Molecular Weight:

calculated_mw: 18.4 kDa

observed_mw: 20 kDa

UniProt: Q8CIZ8