CEACAM5 Protein (AA 323-500) (His tag)

Catalog No. ABIN7827953

Recombinant CEACAM5 protein expressed in HEK-293 Cells.

Quick Overview for CEACAM5 Protein (AA 323-500) (His tag) (ABIN7827953)

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 323-500

Purification tag / Conjugate: This CEACAM5 protein is labelled with His tag.

Purpose: Human CEACAM-5/CD66e Protein, Ultra Low Endotoxin

Sequence: Pro323-Leu500

Characteristics: Recombinant Human CEACAM-5/CD66e (323-500) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro323-Leu500.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.01 EU per μg by the LAL method.

Biological Activity Comment: Human CEACAM-5 (323-500) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CEACAM-5 (323-500) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Alternative Name: CEACAM-5

Background: Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.

Molecular Weight: 20.73 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

UniProt: P06731