IgE Protein (Biotin)
Quick Overview for IgE Protein (Biotin) (ABIN7827980)
Target
See all IgE ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This IgE protein is labelled with Biotin.
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Specificity
- Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown
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Sensitivity
- Reactivity > 99 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)
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Characteristics
- IGHE, chromosome 14q32.33 Source: Human B cell hybridoma from healthy donors (CD40 cell culture system)
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Purification
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - mouse anti-human IgE mAb (BSW17)
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IgE (AA 209-428) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)Origin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-428) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-432) protein (His tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-429) protein (His tag)Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
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Application Notes
- For functional cellular assays (mast cell / basophil activation)
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- PBS, pH=7.2, azide free
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Preservative
- Azide free
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Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
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Storage
- 4 °C
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- IgE
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Background
- Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells
Target
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