CXCR2 Protein (AA 1-63) (Fc Tag)

Catalog No. ABIN7829761

Recombinant CXCR2 protein expressed in HEK-293 Cells.

Quick Overview for CXCR2 Protein (AA 1-63) (Fc Tag) (ABIN7829761)

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Application: Screening Assay (ScA), Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 1-63

Purification tag / Conjugate: This CXCR2 protein is labelled with Fc Tag.

Purpose: Recombinant Cynomolgus CXCR2(1-63) protein with C-terminal human Fc tag

Specificity: CXCR2(Met1-Lys63) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Alternative Name: CXCR2

Background: CD182, IL8R2, IL8RA, IL8RB, CMKAR2, WHIMS2, CDw128b
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

Molecular Weight: predicted molecular mass of 33.4 kDa after removal of the signal peptide.

Pathways: cAMP Metabolic Process