IgE Protein (AA 106-427) (His tag)
Quick Overview for IgE Protein (AA 106-427) (His tag) (ABIN7829857)
Target
See all IgE ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 106-427
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Purification tag / Conjugate
- This IgE protein is labelled with His tag.
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Purpose
- Recombinant human IGHE(106-427) Protein with C-terminal 10xHis tag
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Specificity
- IGHE(Ser106-Gly427) 10xHis tag
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Characteristics
- Extracellular Domain Protein
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IgE (AA 209-428) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)Origin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-428) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-432) protein (His tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-429) protein (His tag)Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
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Application Notes
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Extracellular Domain Proteins (ECD) can be used as:
- Immunogens for antibody drug development
- Reagents used for CAR-T positive cell monitoring
- Reagents for antibody screening and functional testing
- Reagents for antibody affinity measurement
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Comment
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The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please see Certificate of Analysis for specific instructions of reconstitution.
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Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- IgE
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Alternative Name
- IgHE
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Background
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IgE
Enables immunoglobulin receptor binding activity. Involved in several processes, including B cell mediated immunity, Fc receptor-mediated immune complex endocytosis, and leukocyte degranulation. Located in extracellular space and plasma membrane. Part of IgE B cell receptor complex. [provided by Alliance of Genome Resources, Apr 2025] -
Molecular Weight
- predicted molecular mass of 37.1 kDa after removal of the signal peptide. The apparent molecular mass of IGHE(106-427)-His is 35-55 kDa due to glycosylation.
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UniProt
- P01854
Target
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