EML4-ALK (Active) protein (GST tag)

Catalog No. ABIN7856246

Recombinant EML4-ALK protein expressed in Baculovirus infected Insect Cells.

Quick Overview for EML4-ALK (Active) protein (GST tag) (ABIN7856246)

Target: EML4-ALK

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Kinase Activity Assay (KAA)

Product Details

Purification tag / Conjugate: GST tag

Purpose: Recombinant EML4-ALK (Eex20Aex20), Active

Sequence: EML4 (1-580) - ALK (1058-end)

Characteristics: Recombinant human fusion protein EML4 (1-580a.a., exons1-20) - ALK (1058-end, exons20-29) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Sterility: Sterile

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, 10-25 % glycerol

Preservative: Dithiothreitol (DTT), PMSF

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Expiry Date: 12 months

Target Details

Target: EML4-ALK

Sub Type: Fusionprotein

Background: ALK or CD246 is a receptor tyrosine kinase, which belongs to the insulin receptor superfamily which comprises an extracellular domain, a hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. CD246 plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. ALK-positive neoplasms represent a distinct entity because the morphology of the tumors is often neither anaplastic nor large cell and the tumors should be referred to as ALK lymphomas. CD246 has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. EML4-ALK mutations in lung cancer confer resistance to ALK inhibitors.

Molecular Weight: ~165 kDa

NCBI Accession: NM_019063, NM_004304