FAM131B-BRAF (Active) protein (GST tag)

Catalog No. ABIN7856311

This Recombinant FAM131B-BRAF protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for FAM131B-BRAF (Active) protein (GST tag) (ABIN7856311)

Target: FAM131B-BRAF

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Kinase Activity Assay (KAA)

Product Details

Purification tag / Conjugate: GST tag

Purpose: Recombinant FAM131B-BRAF (Fex2Bex9), Active

Sequence: FAB131B (1-30 exon2)-BRAF (381-end or exon9-18)

Characteristics: Recombinant human fusion FAB131B (1-30 exon2)-BRAF (381-end or exon9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Sterility: Sterile

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, 10-25 % glycerol

Preservative: Dithiothreitol (DTT), PMSF

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Expiry Date: 12 months

Target Details

Target: FAM131B-BRAF

Sub Type: Fusionprotein

Background: KIAA1549-BRAF is a gene fusion resulting from a tandem duplication event involving the BRAF kinase gene that have recently been identified as the most frequent genetic alteration in many cancers. The KIAA1549-BRAF fusion typically results from a 2.0 Mb tandem duplication in chromosome band 7q34. The KIAA1549:BRAF fusion gene is considered a driver genetic event in pilocytic astrocytoma and many other pediatric brain neoplasms. KIAA1549-BRAF fusion gene and BRAF (V600E) mutation may be responsible for deregulation of the Ras-RAF-ERK signaling pathway in many brain cancers.

Molecular Weight: ~75 kDa

NCBI Accession: NM_004333