HIV-2 gp36 protein (His tag)

Catalog No. ABIN810024

This Recombinant protein is expressed in Yeast (Pichia pastoris).

Quick Overview for HIV-2 gp36 protein (His tag) (ABIN810024)

Target: HIV-2 gp36

Protein Type: Recombinant

Origin: Human

Source: Yeast (Pichia pastoris)

Application: ELISA, Western Blotting (WB)

Product Details

Purification tag / Conjugate: His tag

Characteristics: Human Immunodeficiency Virus Type 2 (HIV-2) gp36, Recombinant ecto-domain of HIV-2 gp36. Contains a 6 histidine fusion partner. Reacts with HIV-2 positive human sera and monoclonal antibodies as determined by ELISA and Western blot. Major immunospecific band at 14 kDa, minor bands at 5, 16, 18, 28 and 42 kDa.

Purification: Purity determined by SDS-PAGE and Western blot

Application Details

Application Notes: Suitable for use in ELISA and Western blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. HIV-2 gp36 ELISA with HIV-2 positive sera at 1:2.000 with 1-100 nanograms per well gp36. Background with BSA or normal sera OD405 <0.1. Alkaline phosphatase substrate for 45 min.

Comment:

Infectious Disease Antigens and Toxins

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 6 M Urea, 0.02 M Tris-HCl, 0.5 M Sodium chloride, pH 7.0 to 8.0 at room temperature

Preservative: Without preservative

Handling Advice: Aliquot to avoid multiple freeze thaws.

Storage: -80 °C

Storage Comment: Store at -70 °C or below.

Target Details

Target: HIV-2 gp36

Target Type: Viral Protein

Molecular Weight: 14 kDa