Stabilized Troponin I Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
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- Target
- Stabilized Troponin I
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Characteristics
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Purified recombinant Human Stabilized Troponin I protein (Cardiac)
Expression System: E.coli
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Concentration
- 10-50 µg/mL
- Buffer
- Supplied in 500 mM NaCl, 5 mM sodium phosphate, 2 mM CaCl2, 0.5 mM EDTA, 0.025 % Sodium Azide, pH 7.5.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Store at -25 °C or below.
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- Target
- Stabilized Troponin I
- Background
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Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.
Alternative Names: Cardiac-specific troponin I protein, cTnl protein, TnI protein, Troponin I protein
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